Digene's Hybrid Capture 2 is an example of which type of assay?

• A. Solid-phase hybridization assay
• B. Gel electrophoresis-based assay
• C. Mass spectrometry-based assay
• D. Real-time PCR assay

Answer: (A)

The main concept is recognizing a solid-phase hybridization approach used to detect a specific DNA target. Digene’s Hybrid Capture 2 works by pairing target HPV DNA with complementary RNA probes to form DNA–RNA hybrids. These hybrids are then captured on a solid surface and detected with antibodies that recognize DNA–RNA hybrids, usually producing a chemiluminescent signal. This setup relies on hybridization onto a solid phase with antibody-based detection, not on separating fragments, measuring masses, or amplifying DNA in real time. That’s why it’s described as a solid-phase hybridization assay.